Multiply the fold number (2) with the number that is to the right of the colon under the previous test tube (2 x 4 8). Multiply the fold number (2) with the number that is to the right of the colon under the first test tube (2 x 2 4).ĭILUTION PROBLEM ONE:Prepare a serial 2 fold dilution to 1:64 Draw another test tube.
Inside the second test tube, write the same number for total volume as you did for the first test tube (1ml).Īre we done yet No, the number to the right of the colon has to get to 64. Write 1ml above an arrow going into the second test tube, and repeat this for each additional test tube we will do. To find the number to the right of that colon, multiply the fold number (2) with the number that is to the right of the colon under the first test tube (2 x 2 4). Write the fold number (2) to the right of the colon under the test tube.ĭILUTION PROBLEM ONE:Prepare a serial 2 fold dilution to 1:64 Draw a second test tube. Take the number to the right of the colon (2) and subtract by the number of ml going into the test tube (1) to get the total volume of the inside of the test tube (2-1 1 ml total volume).ĭILUTION PROBLEM ONE:Prepare a serial 2 fold dilution to 1:64 Draw a test tube. How many fold does the problem say Write that fold number (2) to the right of the colon under the test tube.
